環境科学研究室 ( ENVIRONMENTAL SCIENCES )
| 教授 | 原田 健一（Professor Ken-ichi Harada, Ph.D.） |
|---|---|
| 助教 | 今西 進（Assistant Professor Susumu Imanishi, Ph.D.） |
| ① | 湖沼生態系におけるラン藻類制御法の確立 |
|---|---|
| ② | 環境微生物を用いる環境浄化法の確立 |
| ③ | 質量分析による生体内タンパク質翻訳後修飾の網羅的解析（PTM proteomics） |
| Sandra Soderholm, Denis E. Kainov, Tiina Ohman, Oxana V. Denisova, Bert Schepens, Evgeny Kulesskiy, Susumu Y. Imanishi, Garry Corthals, Petteri Hintsanen, Tero Aittokallio, Xavier Saelens, Sampsa Matikainen, and Tuula A. Nyman, Phosphoproteomics to characterize host response during influenza A virus infection of human macrophages, Mol. Cell. Proteomics, 15, 3203-3219 (2016). |
| Susumu Y. Imanishi, Takuma Nakayama, Hirohiko Asukabe, and Ken-ichi Harada, Application of MALDI Biotyper to cyanobacterial profiling, Rapid Commun. Mass Spectrom. (Epub ahead of print). |
| Niina M. Santio*, Sebastian K.-J. Landor*, Laura Vahtera, Jani Ylä-Pelto, Elina Paloniemi, Susumu Y. Imanishi, Garry Corthals, Markku Varjosalo, Ganesh babu Manoharan, Asko Uri, Urban Lendahl, Cecilia Sahlgren, and Päivi Koskinen (*equal contribution), Phosphorylation of Notch1 by Pim kinases promotes oncogenic signaling in breast and prostate cancer cells,
Oncotarget, 7, 43220-43238 (2016). |
| 有井鈴江、冨田浩嗣、辻 清美、原田健一、2013年相模湖で起きた溶藻現象時のラン藻類の変化、
名城大学総合研究所総合学術研究論文集(in press). |
| K. Tomita, M. Hasegawa, S. Arii, K. Tsuji, B. Bober, K.-I. Harada, Characteristic Oxidation Behavior of β-Cyclocitral from the Cyanobacterium Microcystis,
Environ. Sci. Poll. Res., 23, 11998–12006 (2016). |
| Elizabeth V. Nguyen, Susumu Y. Imanishi, Pekka Haapaniemi, Avinash Yadav, Markku Saloheimo, Garry L. Corthals, and Tiina M. Pakula, Quantitative site-specific phosphoproteomics of Trichoderma reesei signaling pathways upon induction of hydrolytic enzyme production,
J. Proteome Res., 15, 457-467 (2016). |
| Andrea Roxanne J. Anas, Anna Nakajima, Chiaki Naruse, Mineka Tone, Hirohiko Asukabe, and Ken-ichi Harada, Determination of FVIIa-sTF inhibitors in toxic microcystis cyanobacteria by LC-MS technique, Mar. Drugs., 14, 7 (2016). |
| Nathalie Eisenhardt*, Viduth K. Chaugule*, Stefanie Koidl, Mathias Droescher, Esen Dogan, Jan Rettich, Päivi Sutinen, Susumu Y. Imanishi, Kay Hofmann, Jorma J. Palvimo, and Andrea Pichler (*equal contribution), A new vertebrate SUMO enzyme family reveals insights into SUMO-chain assembly,
Nat. Struct. Mol. Biol., 22, 968-975 (2015). |
| Tomoyasu Nishizawa, Yasuko Neagari, Takamasa Miura, Munehiko Asayama, Koichi Murata, Ken-Ichi Harada and Makoto Shirai, Molecular analysis of the cyanobacterial community in gastric contents of egrets with symptoms of steatitis, Open.
Microbiol. J., 9, 160-166 (2015). |
| Otto Kauko, Teemu Daniel Laajala, Mikael Jumppanen, Petteri Hintsanen, Veronika Suni, Pekka Haapaniemi, Garry Corthals, Tero Aittokallio, Jukka Westermarck, and Susumu Y. Imanishi, Label-free quantitative phosphoproteomics with novel pairwise abundance normalization reveals synergistic RAS and CIP2A signaling,
Sci. Rep., 5, 13099 (2015). |
| Claire L. Hyder*, Kati Kemppainen*, Kimmo O. Isoniemi, Susumu Y. Imanishi, Hidemasa Goto, Masaki Inagaki, Elnaz Fazeli, John E. Eriksson, and Kid Törnquist (*equal contribution). Sphingolipids inhibit vimentin-dependent cell migration,
J. Cell Sci., 28, 2057-2069 (2015). |
| Reetta Virtakoivu, Anja Mai, Elina Mattila, Nicola De Franceschi, Susumu Y. Imanishi, Garry Corthals, Riina Kaukonen, Markku Saari, Fang Cheng, Elin Torvaldson, Veli-Matti Kosma, Arto Mannermaa, Ghaffar Muharram, Christine Gilles, John Eriksson, Ylermi Soini, James B. Lorens, and Johanna Ivaska, Vimentin-ERK signaling uncouples Slug gene regulatory function,
Cancer Res., 75, 2349-2362 (2015). |
| Julia Lindqvist, Susumu Y. Imanishi*, Elin Torvaldson*, Marjo Malinen, Mika Remes, Fanny Örn, Jorma J. Palvimo, and John E. Eriksson (*equal contribution), Cyclin-dependent kinase 5 acts as a critical determinant of AKT-dependent proliferation and regulates differential gene expression by the androgen receptor in prostate cancer cells,
Mol. Biol. Cell, 26, 1971-1984 (2015). |
| Atsushi Miyachi, Fumio Kondo, Miki Kurita, Kiyomi Tsuji, and Ken-ichi Harada, Microbial degradation of linear peptides by strain B-9 of Sphingosinicella and its application in peptide quantification using liquid chromatography-mass spectrometry,
J. Biosci. Bioeng., 119, 724-728 (2015). |
| Darshana Kottahachchi, Lallindra Gooneratne, Anil Jayasekera, Dorota Muth-Pawlak, Robert Moulder, Susumu Y. Imanishi, Ari Ariyaratne, Anne Rokka, and Garry L. Corthals, Quantitative analysis of the erythrocyte membrane proteins in polycythemia vera patients treated with hydroxycarbamide,
EuPA Open Proteomics, 7, 43-53 (2015). |
| Veronika Suni*, Susumu Y. Imanishi*, Alessio Maiolica, Ruedi Aebersold, and Garry L. Corthals (*equal contribution), Confident site localization using a simulated phosphopeptide spectral library,
J. Proteome Res., 14, 2348-2359 (2015). |
| S. Arii, K. Tsuji, K. Tomita, M. Hasegawa, B. Bober, and K.-I. Harada, Cyanobacterial Blue Color Formation during Lysis Process under Natural Conditions,
Appl. Environ. Microbiol., 81, 2667-2675 (2015). |
| A. Miyachi, T. Murase, Y. Yamada, T. Osonoi and K.-I. Harada, A Quantitative Analytical Method for Determining the Levels of Gastric Inhibitory Polypeptides GIP1-42 and GIP3-42 in Human Plasma Using LC-MS/MS/MS,
J. Proteome. Res., 12, 2690-2699 (2013). |
| F. Kuriyama, E. K. Kuroda, K. B. Guergoletto, S. Garcia, G. M. A. de Nobrega, K. Tsuji, K.-I. Harada and E. Y. Hirooka, Biodegradation potential of microcystin by microorganisms,
Eng. Sanit. Ambient., 17, 181-186 (2012). |
| K. Nakanishi, M. Katagi, K. Zaitu, N. Shima, H. Kamata, A. Miki, H. Kato, K.-I. Harada, H. Tsuchihashi and K. Suzuki, Simultaneous Enantiomeric Determination of MDMA and Its Phase I and phase II Metabolites in Urine by Liquid Chromatography-Tandem Mass Spectrometry with Chiral Derivatization,
Anal. Bioanal, Chem. 404, 2427–2435 (2012). |
| 飯田耕太郎、原 脩、灘井雅行、亀井浩行、吉田 勉、秋田谷龍男、打矢恵一、橋爪清松、原田健一、薬剤師国家試験対策の教育支援を目指した学内試験の調査に関する考察、名城大学総合研究所総合学術研究論文集、
11, 47-56 (2012). |
| M. Hasegawa, A. Nishizawa, K. Tsuji, S. Kimura and K.-I. Harada, Volatile Organic Compounds Derived from 2-Keto-acid Decarboxylase in Microcystis aeruginosa, Microbes Environ., 27 , 525-528 (2012). |
| F. Kondo, S. Okada, A. Miyachi, M. Kurita, K. Tsuji and K.-I. Harada, Microbial Degradation of Physiologically Active Peptides by B-9 Strain,
Anal. Bioanal, Chem. 403, 1783-1791 (2012). |
| E. H. Hashimoto, M. Kamogae, T. P. Vanzella, I. M. S. Colus, A. P. F. R. L. Bracarense, M. C. Bittencourt-Oliveira, E. Itano, E. K. Kuroda, H. Kato, S. Nagata, Y. Ueno, K.-I. Harada and E. Y. Hirooka, Biomonitoring of microcystin and aflatoxin co-occurrence in aquaculture using immunohistochemistry and genotoxicity assays (micronucleus and comet assay).
Brazilian Archives of Biology and Techonology, 55,151-159 (2012). |
|
A. R. Coelho, G. M. A. Nóbrega, F. C. Pagnocca, F. L. Hoffmann, K.-I. Harada, E. Y. Hirooka, Evaluation of potential antagonistism in yeasts, seeking biocontrol of spoilage by Penicillium expansum, Semina:
Ciências Agrárias, 32, suplemento 1, 1879-1892, (2011). |
| Yasuko Neagari, Suzue Arii, Mai Udagawa, Manabu Onuma, Yoshiya Odaya, Takeshi Kawasaki, Makio Tenpaku, Hisayo Hayama, Ken-ichi Harada, Masaya Mizukami, and Koichi Murata：Steatitiis in egrets and herons steatitiis in egrets and herons from Japan
J. Wildl. Dis., 47, 49–55 (2011) |
| Tomoyasu Nishizawa, Akiko Ueda, Tomoyo Nakano, Akito Nishizawa, Takamasa Miura, Munehiko Asayama, Kiyonaga Fujii, Ken-ichi Harada, and Makoto Shirai：Characterization of the locus of genes encoding enzymes producing heptadepsipeptide micropeptin in the unicellular cyanobacterium Microcystis.
J. Biochem., 149, 475–485 (2011) |
| Azusa Kato, H. Hirata, Yositami Ohhashi, Kiyonaga Fujii, Kenji Mori, Ken-ichi. HaradaA new anti-MRSA antibiotic complex, WAP-8294A II. Structure Characterization of Minor Components by Electrospray Ionization LCMS and MS/MS
J. Antibiotics, 64, 373-379 (2011) |
| Ken-ichi Harada, Hajime Kato, Kiyomi Tsuji: Antimicrobial Activity of Various Antibiotics against B-9 Strain That Degrades Microcystin Produced by Cyanobacteria
J. Res. Insti. Meijo Univ., 10, 35-43 (2011) |
| Salfarina Ramli, K.-I. Harada, Nijsiri Ruangrungsi: Antioxidant, Antimicrobial and Cytotoxicity Activities of Acacia farnesiana (L.) Willd. Leaves Ethanolic Extract
Pharmacognocy J., 3, 50-58 (2011) |
| Hideaki Yamaguchi, Tao Yu, Toshiro Noshita, Yumi Kidachi, Katsuyoshi Kamiie, Kenji Yoshida, Tatsuo Akitaya, Hironori Umetsu, and Kazuo Ryoyama：Ligand-receptor interaction between triterpenoids and the 11β-hydroxysteroid dehydrogenase type 2 (11βHSD2) enzyme predicts their toxic effects against tumorigenic r/m HM-SFME-1 cells
J. Biol. Chem., 286, 36888-36897 (2011) |
| Hideaki Yamaguchi, Tao Yu, Yumi Kidachi, Tatsuo Akitaya, Kenji Yoshida, Katsuyoshi Kamiie, Toshiro Noshita, Hironori Umetsu, and Kazuo Ryoyama：Selective toxicity of glycyrrhetinic acid against tumorigenic r/m HM-SFME-1 cells is potentially attributed to downregulation of glutathione
Biochimie., 93, 1172-1178 (2011) |
| Hideaki Yamaguchi, Tatsuo Akitaya, Tao Yu, Yumi Kidachi, Katsuyoshi Kamiie, Toshiro Noshita, Hironori Umetsu, and Kazuo RyoyamaHomology modeling and structural analysis of 11β-hydroxysteroid dehydrogenase type 2
Eur. J. Med. Chem., 46, 1325-1330 (2011) |
| Ken-ichi Harada, Masateru Hasegawa, Naoko Fukushima, Akito Nishizawa, Daiki Fujise, and Kiyomi Tsuji：Degradation behavior of β -carotene during cultivation of cyanobacteria
J. Res. Insti. Meijo Univ., 9, 83–91 (2010) |
| Daiki Fujise, Kiyomi Tsuji, Naoko Fukushima, Kohei Kawai, and Ken-ichi Harada：Analytical aspects of cyanobacterial volatile organic compounds for investigation of their production behavior
J. Chromatogr. A, 1217, 6122–6125 (2010) |
| Takuya Kumamoto, Hiroyuki Koshino, Daisuke Watanabe, Yuko Matsumoto, Kazuki Aoyama, Ken-ichi Harada, Tsutomu Ishikawa, and Yuzuru Mikami：M10709, a novel cyclic peptide antibiotic from clinically isolated Streptomyces sp.
Heterocycles., 80, 281-288 (2010) |
| Hideaki Yamaguchi, Toshiro Noshita, Tao Yu, Yumi Kidachi, Katsuyoshi Kamiie, Hironori Umetsu, and Kazuo RyoyamaHideaki Yamaguchi, Toshiro Noshita, Tao Yu, Yumi Kidachi, Katsuyoshi Kamiie, Hironori Umetsu, and Kazuo Ryoyama
Eur. J. Med. Chem., 45, 2943-2948 (2010) |
| Hajime Kato, Kiyomi Tsuji, and Ken-ichi Harada：Microbial Degradation of cyclic peptides produced by bacteria
J. Antibiotics., 62, 181-190 (2009) |
| Takahiko Noguchi, Azusa Shinohara, Akito Nishizawa, Munehiko Asayama, Tomoyo Nakano, Masateru Hasegawa, Ken-ichi Harada, Tomoyasu Nishizawa, and Makoto Shirai：Genetic analysis of the microcystin biosynthesis gene cluster in Microcystis strains from four bodies of eutrophic water in Japan
J. Gen. Appl. Microbiol., 55, 111-123 (2009) |
| Daisuke Watanabe, Rie Kerakawati, Tetsuo Morita, Tomonori Nakamura, Koichi Ueno, Takaya Kumamoto, Waka Nakanishi, Tsutomu Ishikawa, Jun Uzawa, Hiriko Seki, Masahiko Tachi, Ken-ichi Harada, Yoshinori Higuchi, and Chaiyo Chaichantipyuth：Isolation of β-sitosterol and digalactopyranosyl- diacylglyceride from Ciraus hystrix, a Thai traditional herb, as pancreatic lipase inhibitors
Heterocycles, 78, 1497-1505 (2009) |
| Keiko Ozaki, Emiko Ito, Saori Tanabe, Kaori Natsume, Kiyomi Tsuji, and Ken-ichi Harada：Electron microscopic study on lysis of a cyanobacterium Microcystis
J. Health Sci., 55, 578-585 (2009) |
| Akito Nishizawa, Tomoyasu Nishizawa, Munehiko Asayama, Masateru Hasegawa, Ken-ichi Harada and Makoto Shirai：Correlation between variation of McyB1 module and biosynthesis of microcystin isoforms.
J. Res. Insti. Meijo Univ., 8, 53-62 (2009) |
| Akira Mukai, Toshio Fukai, Yasutaka Hoshino, Katsukiyo Yazawa, Ken-ichi Harada, and Yuzuru Mikami：Nocardithiocin, a novel thiopeptide antibiotic, produced by pathogenic Nocardia pseudobrasiliensis IFM 0757
J. Antibiotics., 62, 613-619 (2009) |
| Ken-ichi Harada, Keiko Ozaki, S. Tsuzuki, Hajime Kato, Masateru Hasegawa, Emilia K. Kuroda, Suzuw Arii and Kiyomi Tsuji：Blue color formation of cyanobacteria with β-cyclocitral
J. Chem. Ecol., 35, 1295-1301 (2009) |
| Alexandre Rodrigo Coelho, Masahiko Tachi, Fernando Carlos Pagnocca, Gisele Maria Andrade Nóbrega, Fernando Leite Hoffmann, Ken-ichi Harada, and Elisa Yoko Hirooka：Purification of Candida guilliermondii and Pichia ohmeri killer toxin as active agent against Penicillium expansum
Food Additives and Contaminants, 26, 73–81 (2009) |
| Elisabete Hiromi Hashimoto, Hajime Kato, Yoshito Kawasaki, Yuriko Nozawa, Kiyomi Tsuji, Elisa Yoko Hirooka, and Ken-ichi Harada：Further investigation of microbial degradation of microcystin using advanced Marfey’s method
Chem. Res. Toxicol., 22, 391–398 (2009) |
| Yuriko Nozawa, Akira Kawashima, Elisabete Hiromi Hashimoto, Hajime Kato, and Ken-ichi Harada：Application of Log D for the Prediction of Hydrophobicity in Advanced Marfey’s Method
J. Chromatogr. A., 1216, 3807–3811 (2009) |
| Yuriko Nozawa, Ken-ichi Harada, Akemi Ryoda, Takuya Kumamoto, Waka Nakanishi, and Tutomu Ishikawa：Optical resolution of 1,2-diamino compounds using advanced Marfey’s method
J. Mass Spectrom. Soc. Jpn., 57, 71–74 (2009) |
| 原田健一：マイクロシスチン、薬毒物分析学辞典（高取健彦、黒岩幸雄、Anthony T. Tu、山本郁男 編集）
pp.232-233 廣川書店（平成21年9月） |
| 原田健一、供田 洋：微生物が生み出す医薬品、化学療法薬 病原微生物・がんと戦う（上野芳夫、大村 智監修、田中晴雄、土屋友房編集）
pp.263–272 南江堂（平成21年3月） |